Published Online
on January 26, 2009

A more recent version of this article appeared on March 1, 2009

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Original Article

Evaluation of a Novel 18F-Labeled PET Perfusion Tracer for the Assessment of Myocardial Infarct Size in Rat

Hossam M. Sherif^1,3; Antti Saraste¹; Eliane Weidl¹; Axel W. Weber¹; Takahiro Higuchi¹; Sybille Reder¹; Thorsten Poethko¹; Gjermund Henriksen¹; David Casebier²; Simon Robinson²; Hans-Jürgen Wester¹; Stephan G. Nekolla¹ and Markus Schwaiger¹

¹ Klinikum Rechts der Isar, Technischen Universität München, Munich, Germany;
² Lantheus Medical Imaging, North Billerica, Massachusetts

³ E-mail: hossam_sherif_66{at}hotmail.com

Background—Evaluation of a new 18F-Labeled PET perfusion tracer; 18F BMS747158-02, for the assessment of myocardial infarct (MI) size.

Methods and Results—Wistar rats were studied 24 hours after ligation of the left coronary artery (LCA) either permanently (n=15) or transiently (n=16) for 30 minutes. Seven non-operated rats were studied as controls. The rats were injected with 37 MBq of 18F BMS747158-02 and imaged with a small animal PET scanner for 20 minutes. Polar maps were generated for measurement of PET defect size, and left ventricular (LV) systolic and diastolic volumes were assessed in gated images. As a reference, MI size was determined by 2,3,5-triphenyltetrazolium chloride (TTC) staining of LV tissue samples. Permanent or transient ligation of the LCA produced transmural or subendocardial MI of variable sizes, respectively. In normal rats, PET imaging demonstrated intense and homogeneous uptake of 18F BMS747158-02 throughout the myocardium. After ligation, sharply defined perfusion defects were present. Throughout the imaging period, the defect size correlated closely with the MI size either after permanent (r=0.88, p<0.01, mean difference 1.86%) or transient (r=0.92, p<0.01, mean difference 2.16%) ligation of the LCA. Moreover, reduction of LV systolic function measured with PET correlated with the MI size (r=-0.81, p<0.01, n=23).

Conclusions—Myocardial 18F BMS747158-02 PET imaging provides excellent image quality and uptake properties enabling accurate evaluation of MI size and LV function in rat. It is a promising technique for evaluation of MI size in clinical trials.

Key Words: 18F BMS747158-02 • TTC staining • cardiac PET • myocardial infarction • perfusion

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Evaluation of a Novel ¹⁸F-Labeled Positron-Emission Tomography Perfusion Tracer for the Assessment of Myocardial Infarct Size in Rats

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